Recipes

How To Make 0.5M EDTA pH 8.0

About EDTA solution Ethylenediaminetetraacetic acid (EDTA) solution, at 0.5 M pH 8.0, is a commonly used solution utilised as a ligand and chelating agent. EDTA...

How To Make TE Buffer pH 8.0

About TE buffer TE buffer (Tris-EDTA) is a commonly used buffer solution for resuspending and storing nucleic acids, especially DNA. The Tris solution keeps the...

How To Make 1 M Tris-HCl pH 8.0 Buffer

About Tris-HCl buffer solution Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) is a commonly used buffering solution. The role of Tris-HCl in molecular biology is to control the acidity...

How To Make TBE Buffer

About TBE buffer TBE buffer, named so because of the three ingredients of Tris base, Boric acid and EDTA, is a solution commonly used as...

How To Make 10% SDS Stock Solution

About SDS solution Sodium dodecyl sulfate (SDS) is a detergent commonly used in lysis buffers to aid in permeabilising cell membranes and in polyacrylamide gel...

How To Make LB Agar Plates

About LB agar plates Luria-Bertani (LB) agar plates are nutrient-rich plates used for growing bacteria on. They are commonly used during cloning experiments to amplify...

How To Make LB Media

About LB media Luria-Bertani (LB) is a nutrient-rich liquid broth for growing bacteria. It is commonly used during cloning experiments to amplify competent bacteria. When...

How To Make TAE Buffer

About TAE buffer TAE (Tris-acetate-EDTA) buffer, named so because of the three ingredients of Tris base, Acetic acid and EDTA, is a solution commonly used...

How To Make 1x Phosphate Buffered Saline (PBS)

About phosphate buffered saline solution Phosphate buffered saline (PBS) solution is a commonly used buffer used in a variety of biological applications in order to...

How To Make 5 M Sodium Chloride Solution

About sodium chloride solution Sodium chloride (NaCl) solution, especially at a 5 M concentration, is a commonly used stock solution for a variety of solutions....