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# Statistics & Maths

### How To Perform A Non-Parametric Partial Correlation In SPSS

Partial correlations are great in that you can perform a correlation between two continuous variables whilst controlling for various confounders. However, the partial correlation...

### How To Calculate PCR Primer Efficiencies

Why bother with PCR primer efficiencies? Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain...

### How To Calculate The Coefficient Of Variation (CV)

What is the coefficient of variation? The coefficient of variation (CV) is a measure of precision from repeated measures. Within the lab, it is mainly...

### How To Create A Linear Standard Curve In Excel

In this guide I will explain how to create a linear standard curve using Microsoft Excel and how to use it to calculate unknown...

### How To Perform The Pfaffl Method For qPCR

What is the Pfaffl method? The Pfaffl method, named after it's curator Michael Pfaffl, is used to calculate relative gene expression data while accounting for...

### How To Perform A Mann-Whitney U Test In SPSS

What is a Mann-Whitney U test? A Mann-Whitney U test is a non-parametric alternative to an unpaired (independent) t-test to determine the difference between two...

### How To Use The Standard Error Formula

In this guide I will explain what the standard error formula is and how to use it to work out the standard error through...

### How To Test Data For Normality In SPSS

The very first thing you should do before performing any statistical test, is to see whether your data is normally distributed. Normality testing in...

### How To Split A File In SPSS

What is the Split File command? The Split File command is used to separate the output of SPSS tests according to a group variable. The...

### How To Dilute New PCR Primers

So you have designed and ordered your primers and now they have finally arrived. But how do you prepare lyophilised (freeze-dried powder form) oligo...