### How To Calculate PCR Primer Efficiencies

Why bother with PCR primer efficiencies?
Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain...

### How To Calculate The Coefficient Of Variation (CV)

What is the coefficient of variation?
The coefficient of variation (CV) is a measure of precision from repeated measures. Within the lab, it is mainly...

### How To Create A Linear Standard Curve In Excel

In this guide I will explain how to create a linear standard curve using Microsoft Excel and how to use it to calculate unknown...

### How To Perform The Pfaffl Method For qPCR

What is the Pfaffl method?
The Pfaffl method, named after it's curator Michael Pfaffl, is used to calculate relative gene expression data while accounting for...

### How To Use The Standard Error Formula

In this guide I will explain what the standard error formula is and how to use it to work out the standard error through...

### How To Dilute New PCR Primers

So you have designed and ordered your primers and now they have finally arrived. But how do you prepare lyophilised (freeze-dried powder form) oligo...

### The Hardy-Weinberg Equations And How To Use Them

What is the Hardy-Weinberg principle?
The Hardy-Weinberg principle, also referred to as the Hardy-Weinberg equilibrium, is a set of 5 assumptions which when satisfied can...

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