How To Perform The Delta-Delta Ct Method
What is the delta-delta Ct method?
The delta-delta Ct method, also known as the 2-∆∆Ct method, is a simple formula used in order to calculate...
The C1V1 = C2V2 Equation Explained
The simple formula of C1V1 = C2V2 is a lifesaver for bioscience researchers in the lab who are wanting to do dilutions. Here I...
How To Calculate PCR Primer Efficiencies
Why bother with PCR primer efficiencies?
Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain...
How To Calculate The Coefficient Of Variation (CV)
What is the coefficient of variation?
The coefficient of variation (CV) is a measure of precision from repeated measures. Within the lab, it is mainly...
How To Use The Standard Error Formula
In this guide I will explain what the standard error formula is and how to use it to work out the standard error through...
How To Dilute New PCR Primers
In this article, I will explain how to easily prepare PCR stock primers and how to dilute them into a working primer solution.
Mastering qPCR
A...
The Hardy-Weinberg Equations And How To Use Them
What is the Hardy-Weinberg principle?
The Hardy-Weinberg principle, also referred to as the Hardy-Weinberg equilibrium, is a set of 5 assumptions which when satisfied can...
Cohen’s d Calculator: A Quick And Easy Method
Below is the Cohen's d calculator. Simply enter the groups mean and standard deviation values into the calculator, click the calculate button and Cohen's...
How To Analyse qPCR Results With Multiple Reference Genes
A question that I often come across for those who are calculating relative gene expression values in qPCR is, how to go about using...
How To Calculate DNA Copy Number For qPCR
In this article, I will show you how to calculate the DNA copy number for qPCR, by using the known amount and length of...
