Guide To Agarose Gel Electrophoresis
If you are using DNA or doing PCR’s in your research, then you can guarantee you need to make up agarose gels at some...
How To Create Real-Time PCR Primers Using Primer-BLAST
Primer-BLAST
Primer-BLAST is NCBI’s free online primer design platform which designs PCR primers using the Primer3 system and simultaneously checks the likelihood that the primers...
How To Perform The Pfaffl Method For qPCR
What is the Pfaffl method?
The Pfaffl method, named after it's curator Michael Pfaffl, is used to calculate relative gene expression data while accounting for...
What Is A GC Clamp In PCR Primers?
In this article, I will explain what a GC clamp is in relation to PCR primer design and why they may be useful to...
How To Calculate PCR Primer Efficiencies
Why bother with PCR primer efficiencies?
Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain...
The Nanodrop Results Explained
The Nanodrop is one of my favourite pieces of equipment in the lab. In exchange for just 1 μL of sample, not only do...
The Hardy-Weinberg Equations And How To Use Them
What is the Hardy-Weinberg principle?
The Hardy-Weinberg principle, also referred to as the Hardy-Weinberg equilibrium, is a set of 5 assumptions which when satisfied can...
How To Perform The Delta-Delta Ct Method
What is the delta-delta Ct method?
The delta-delta Ct method, also known as the 2-∆∆Ct method, is a simple formula used in order to calculate...
How To Calculate DNA Copy Number For qPCR
In this article, I will show you how to calculate the DNA copy number for qPCR, by using the known amount and length of...
Qubit vs Nanodrop: Which Is Better?
When it comes to quantifying nucleic acids, there are two preferred main platforms: the Qubit and the Nanodrop. Both work slightly differently and have...
