PCR

Find guides, protocols and helpful tips when performing the polymerase chain reaction (PCR).

13 Free PCR Primer Design Programs

Gone are the days where you have to design primers by hand. Instead, we have a plethora of excellent resources available that utilises complex...

How To Calculate PCR Primer Efficiencies

Why bother with PCR primer efficiencies? Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain...
Pfaffl results graph

How To Perform The Pfaffl Method For qPCR

What is the Pfaffl method? The Pfaffl method, named after it's curator Michael Pfaffl, is used to calculate relative gene expression data while accounting for...

How To Make TE Buffer pH 8.0

About TE buffer TE buffer (Tris-EDTA) is a commonly used buffer solution for resuspending and storing nucleic acids, especially DNA. The Tris solution keeps the...

How To Dilute New PCR Primers

So you have designed and ordered your primers and now they have finally arrived. But how do you prepare lyophilised (freeze-dried powder form) oligo...

How To Make TBE Buffer (10x)

About TBE buffer TBE buffer, named so because of the of the three ingredients of Tris base, Boric acid and EDTA, is a solution commonly...
Bisulfite pyrosequencing pyrogram

How Does Bisulfite Pyrosequencing Work?

What is bisulfite pyrosequencing? Bisulfite, also referred to as bisulphite, pyrosequencing is a technique used to quantify DNA methylation levels on DNA at a single-base...

What Is The Polymerase Chain Reaction (PCR)?

What is the Polymerase Chain Reaction (PCR)? PCR is a molecular technique frequently applied to amplify regions of interest in DNA, resulting in millions or...

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