# Statistics & Maths

### How To Calculate PCR Primer Efficiencies

Why bother with PCR primer efficiencies? Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain...

### How To Calculate The Coefficient Of Variation (CV)

What is the coefficient of variation? The coefficient of variation (CV) is a measure of precision from repeated measures. Within the lab, it is mainly...

### How To Create A Linear Standard Curve In Excel

In this guide I will explain how to create a linear standard curve using Microsoft Excel and how to use it to calculate unknown...

### How To Perform A Mann-Whitney U Test In SPSS

What is a Mann-Whitney U test? A Mann-Whitney U test is a non-parametric alternative to the independent (unpaired) t-test to determine the difference between two...

### How To Test Data For Normality In SPSS

The very first thing you should do before performing any statistical test, is to see whether your data is normally distributed. Normality testing in...

### How To Use The Standard Error Formula

In this guide I will explain what the standard error formula is and how to use it to work out the standard error through...

### How To Use The Split File Command In SPSS

What is the Split File command? The Split File command is used to separate the output of SPSS tests according to a group variable. The...

### How To Dilute New PCR Primers

In this article, I will explain how to easily prepare PCR stock primers and how to dilute them into a working primer solution. So you...

### How To Perform A One-Way ANOVA Test In SPSS

This guide will explain, step by step, how to perform a one-way ANOVA test in the SPSS statistical software by using an example. The...

### How To Perform An Unparied T-Test In GraphPad Prism

In this guide, I will explain how to perform an unpaired (independent) T-test in GraphPad Prism. I will also show you how to interpret...