Statistics & Maths

How To Analyse qPCR Results With Multiple Reference Genes

A question that I often come across for those who are calculating relative gene expression values in qPCR is, how to go about using...

How To Perform A One-Sample T-Test In SPSS

What is a one-sample t-test test? A one-sample t-test is a statistical procedure used to determine if there is a significant difference between the sample...

How To Identify And Remove Outliers In GraphPad Prism

In this guide, I will explain how to identify and remove outliers by using GraphPad Prism. To remove or not remove outliers? Outliers are data points...

How To Interpolate A Standard Curve In GraphPad Prism

In this tutorial, I will show you how to interpolate a standard curve by using GraphPad Prism. For this example, I have performed an...

How To Use The Split File Command In SPSS

What is the Split File command? The Split File command is used to separate the output of SPSS tests according to a group variable. The...

How To Perform A Pearson Correlation Test In Excel

In this guide, I will show you how to perform a Pearson correlation test in Microsoft Excel. This includes determining the Pearson correlation coefficient...

What Is A P-Value In Statistics? A Clear Explanation

You have probably heard scientists quote p-values whenever they report the results from their experiment. But, what is a p-value anyway? In this article, I will clearly...

How To Set Up And Enter Data Into RevMan

In this RevMan tutorial, I will explain how to set-up a new file and how to go about entering study data to be able...

How To Perform A Paired T-Test In GraphPad Prism

To perform a paired (dependent) T-test in GraphPad Prism you will need to enter two groups of data into separate columns. Upon opening GraphPad...

How To Calculate PCR Primer Efficiencies

Why bother with PCR primer efficiencies? Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain...