Techniques

How To Create A CpG Assay Using PyroMark Assay Design

To be able to design a CpG assay using the PyroMark Assay Design software, you first need your DNA sequence of interest that contains...
Pfaffl results graph

How To Perform The Pfaffl Method For qPCR

What is the Pfaffl method? The Pfaffl method, named after it's curator Michael Pfaffl, is used to calculate relative gene expression data while accounting for...

How To Make 0.5M EDTA pH 8.0

About EDTA solution Ethylenediaminetetraacetic acid (EDTA) solution, at 0.5 M pH 8.0, is a commonly used solution utilised as a ligand and chelating agent. EDTA...

13 Free PCR Primer Design Programs

Gone are the days where you have to design primers by hand. Instead, we have a plethora of excellent resources available that utilises complex...

How To Analyse ChIP qPCR Data

There are a few ways in which you can analyse chromatin immunoprecipitation (ChIP) data acquired from quantitative real-time polymerase chain reaction (qPCR). Two of...

How To Make TE Buffer pH 8.0

About TE buffer TE buffer (Tris-EDTA) is a commonly used buffer solution for resuspending and storing nucleic acids, especially DNA. The Tris solution keeps the...

How To Dilute New PCR Primers

In this article, I will explain how to easily prepare PCR stock primers and how to dilute them into a working primer solution. So you...

5 Vital Tips For Perfect RNA Extraction Using TRIzol

RNA extraction using TRIzol is one of the most common molecular biology techniques used in the lab. Compared to spin column methods, it requires...
Hardy-Weinberg equation featured image

The Hardy-Weinberg Equations And How To Use Them

What is the Hardy-Weinberg principle? The Hardy-Weinberg principle, also referred to as the Hardy-Weinberg equilibrium, is a set of 5 assumptions which when satisfied can...

How To Make TBE Buffer

About TBE buffer TBE buffer, named so because of the three ingredients of Tris base, Boric acid and EDTA, is a solution commonly used as...