How To Perform The Delta-Delta Ct Method
What is the delta-delta Ct method?
The delta-delta Ct method, also known as the 2-∆∆Ct method, is a simple formula used in order to calculate...
How Does Bisulfite Pyrosequencing Work?
What is bisulfite pyrosequencing?
Bisulfite, also referred to as bisulphite, pyrosequencing is a technique used to quantify DNA methylation levels on DNA at a single-base...
What Is A Cycle Threshold (Ct) Value In qPCR?
In this article, I will explain what is meant by a Ct value in quantification (real-time) PCR (qPCR). I will also discuss the difference...
How To Analyse qPCR Results With Multiple Reference Genes
A question that I often come across for those who are calculating relative gene expression values in qPCR is, how to go about using...
What Is A GC Clamp In PCR Primers?
In this article, I will explain what a GC clamp is in relation to PCR primer design and why they may be useful to...
How To Perform The Pfaffl Method For qPCR
What is the Pfaffl method?
The Pfaffl method, named after it's curator Michael Pfaffl, is used to calculate relative gene expression data while accounting for...
What Percentage Agarose Is Required For Electrophoresis?
In this article, I will explain how an agarose gel works to separate nucleic acids during electrophoresis. Also, I will discuss how to select...
How To Create Real-Time PCR Primers Using Primer-BLAST
Primer-BLAST
Primer-BLAST is NCBI’s free online primer design platform which designs PCR primers using the Primer3 system and simultaneously checks the likelihood that the primers...
What Is ROX And Why Use It In qPCR?
To ROX or not to ROX? That is the question. I frequently see people confused about what ROX is and why it is used...
The Features Of A Good QPCR Primer Pair
In this guide, I will give you some of my thoughts on features of a good qPCR primer pair. I will explain what to...
