How To Calculate DNA Copy Number For qPCR
In this article, I will show you how to calculate the DNA copy number for qPCR, by using the known amount and length of...
DNA Copy Number Online Calculator
Below is a calculator that can be used to estimate the number of copies of DNA, by simply entering the amount used and the...
The Nanodrop Results Explained
The Nanodrop is one of my favourite pieces of equipment in the lab. In exchange for just 1 μL of sample, not only do...
Qubit vs Nanodrop: Which Is Better?
When it comes to quantifying nucleic acids, there are two preferred main platforms: the Qubit and the Nanodrop. Both work slightly differently and have...
What Percentage Agarose Is Required For Electrophoresis?
In this article, I will explain how an agarose gel works to separate nucleic acids during electrophoresis. Also, I will discuss how to select...
How Does Bisulfite Pyrosequencing Work?
What is bisulfite pyrosequencing?
Bisulfite, also referred to as bisulphite, pyrosequencing is a technique used to quantify DNA methylation levels on DNA at a single-base...
How To Make 0.5M EDTA pH 8.0
About EDTA solution
Ethylenediaminetetraacetic acid (EDTA) solution, at 0.5 M pH 8.0, is a commonly used solution utilised as a ligand and chelating agent. EDTA...
How To Make TBE Buffer
About TBE buffer
TBE buffer, named so because of the three ingredients of Tris base, Boric acid and EDTA, is a solution commonly used as...
5 Vital Tips For Perfect RNA Extraction Using TRIzol
RNA extraction using TRIzol is one of the most common molecular biology techniques used in the lab. Compared to spin column methods, it requires...
Load An Agarose Gel In Minutes With Parafilm
When you come to load an agarose gel with your DNA/PCR samples, you will first need to mix them with a loading buffer. The...
