PCR

Find guides, protocols and helpful tips when performing the polymerase chain reaction (PCR).

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How To Calculate DNA Copy Number For qPCR

In this article, I will show you how to calculate the DNA copy number for qPCR, by using the known amount and length of...

DNA Copy Number Online Calculator

Below is a calculator that can be used to estimate the number of copies of DNA, by simply entering the amount used and the...

What Is ROX And Why Use It In qPCR?

To ROX or not to ROX? That is the question. I frequently see people confused about what ROX is and why it is used...
Qubit vs Nanodrop featured image

Qubit vs Nanodrop: Which Is Better?

When it comes to quantifying nucleic acids, there are two preferred main platforms: the Qubit and the Nanodrop. Both work slightly differently and have...

qPCR Primer Efficiency Calculator

Below is a PCR primer efficiency calculator. All that is needed for the calculator is the slope of the standard curve during the serial...

How To Analyse qPCR Results With Multiple Reference Genes

A question that I often come across for those who are calculating relative gene expression values in qPCR is, how to go about using...
Agarose gel comparison different concentrations

What Percentage Agarose Is Required For Electrophoresis?

In this article, I will explain how an agarose gel works to separate nucleic acids during electrophoresis. Also, I will discuss how to select...
Random primers, oligo(dT) and gene-specific primers for cDNA synthesis

Random Primers Or Oligo(dT) Primers For cDNA Synthesis?

Having extracted and assessed the total RNA from your samples, you now have the task of creating the first strand complementary DNA (cDNA). Sounds...
Agarose gel

Reasons Why Your PCR Reaction Does Not Work

We have all been there. You have been performing PCRs perfectly for weeks/months on end but now, for some bizarre reason, you see …...
qPCR amplification plot annotated

What Is A Cycle Threshold (Ct) Value In qPCR?

In this article, I will explain what is meant by a Ct value in quantification (real-time) PCR (qPCR). I will also discuss the difference...

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