Recipes

How To Make 1x Phosphate Buffered Saline (PBS)

About phosphate buffered saline solution Phosphate buffered saline (PBS) solution is a commonly used buffer used in a variety of biological applications in order to...

How To Make 5 M Sodium Chloride Solution

About sodium chloride solution Sodium chloride (NaCl) solution, especially at a 5 M concentration, is a commonly used stock solution for a variety of solutions....

How To Make 3 M Sodium Acetate, pH 5.2

About sodium acetate solution Sodium acetate solution, especially at a 3 M concentration and with a pH of 5.2, is frequently used during DNA and...

How To Make TAE Buffer

About TAE buffer TAE (Tris-acetate-EDTA) buffer, named so because of the three ingredients of Tris base, Acetic acid and EDTA, is a solution commonly used...

How To Make LB Agar Plates

About LB agar plates Luria-Bertani (LB) agar plates are nutrient-rich plates used for growing bacteria on. They are commonly used during cloning experiments to amplify...

How To Make LB Media

About LB media Luria-Bertani (LB) is a nutrient-rich liquid broth for growing bacteria. It is commonly used during cloning experiments to amplify competent bacteria. When...

How To Make 10% SDS Stock Solution

About SDS solution Sodium dodecyl sulfate (SDS) is a detergent commonly used in lysis buffers to aid in permeabilising cell membranes and in polyacrylamide gel...

How To Make 1 M Tris-HCl pH 8.0 Buffer

About Tris-HCl buffer solution Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) is a commonly used buffering solution. The role of Tris-HCl in molecular biology is to control the acidity...

How To Make 0.5M EDTA pH 8.0

About EDTA solution Ethylenediaminetetraacetic acid (EDTA) solution, at 0.5 M pH 8.0, is a commonly used solution utilised as a ligand and chelating agent. EDTA...

How To Make TE Buffer pH 8.0

About TE buffer TE buffer (Tris-EDTA) is a commonly used buffer solution for resuspending and storing nucleic acids, especially DNA. The Tris solution keeps the...

STAY CONNECTED

137FansLike
143FollowersFollow
177SubscribersSubscribe