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Top Tip Bio
Stats & Maths
How To Make RIPA Lysis Buffer
About RIPA lysis buffer RIPA, which stands for radioimmunoprecipitation assay, buffer is a commonly used lysis solution used lyse cells and tissues while preventing protein...
How To Make 1x Phosphate Buffered Saline (PBS)
About phosphate buffered saline solution Phosphate buffered saline (PBS) solution is a commonly used buffer used in a variety of biological applications in order to...
How To Make 5 M Sodium Chloride Solution
About sodium chloride solution Sodium chloride (NaCl) solution, especially at a 5 M concentration, is a commonly used stock solution for a variety of solutions....
How To Make 3 M Sodium Acetate, pH 5.2
About sodium acetate solution Sodium acetate solution, especially at a 3 M concentration and with a pH of 5.2, is frequently used during DNA and...
How To Make TAE Buffer
About TAE buffer TAE (Tris-acetate-EDTA) buffer, named so because of the three ingredients of Tris base, Acetic acid and EDTA, is a solution commonly used...
How To Make LB Agar Plates
About LB agar plates Luria-Bertani (LB) agar plates are nutrient-rich plates used for growing bacteria on. They are commonly used during cloning experiments to amplify...
How To Make LB Media
About LB media Luria-Bertani (LB) is a nutrient-rich liquid broth for growing bacteria. It is commonly used during cloning experiments to amplify competent bacteria. When...
How To Make 10% SDS Stock Solution
About SDS solution Sodium dodecyl sulfate (SDS) is a detergent commonly used in lysis buffers to aid in permeabilising cell membranes and in polyacrylamide gel...
How To Make 1 M Tris-HCl pH 8.0 Buffer
About Tris-HCl buffer solution Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) is a commonly used buffering solution. The role of Tris-HCl in molecular biology is to control the acidity...
How To Make 0.5M EDTA pH 8.0
About EDTA solution Ethylenediaminetetraacetic acid (EDTA) solution, at 0.5 M pH 8.0, is a commonly used solution utilised as a ligand and chelating agent. EDTA...
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