How To Make 1x Phosphate Buffered Saline (PBS)

About phosphate buffered saline solution

Phosphate buffered saline (PBS) solution is a commonly used buffer used in a variety of biological applications in order to maintain the pH of a solution. Since the osmolarity matches that of the human body, PBS is frequently used during cell model washing preparations whilst preventing cell lysis.

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1x phosphate buffered saline recipe

The recipe below can be used to prepare a 1 L 1x PBS solution.

Reagent Weight/Volume Final concentration
Sodium chloride 8.00 grams 137 mM
Potassium chloride 0.20 grams 2.7 mM
Disodium phosphate (Na2HPO4) 1.44 grams 10 mM
Monopotassium phosphate (KH2PO4) 0.24 grams 1.8 mM
MilliQ water Up to 1 L

How to make a 1x PBS solution

  1. Weigh out 8.00 g sodium chloride, 0.20 g potassium chloride, 1.44 g disodium phosphate and 0.24 g monopotassium phosphate and add to a 1 L Duran bottle.
  2. Measure out 800 mL of MilliQ water and add to the Duran bottle.
  3. Dissolve the reagents by adding a magnetic flea into the bottle and placing on a magnetic stirrer.
  4. Adjust the pH to 7.4 with hydrochloric acid (HCl).
  5. Top up the solution to 1 L with MilliQ water.
  6. To sterilise, autoclave the solution on a liquid cycle (20 min at 15 psi).

Storage of 1x PBS solution

Store 1x PBS solution at room temperature (+15oC – +25oC).

Safety

PBS is not classified as hazardous. However, always be sure to read the safety data sheet before use.

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