About sodium acetate solution
Sodium acetate solution, especially at a 3 M concentration and with a pH of 5.2, is frequently used during DNA and RNA extractions. Since sodium acetate is a salt, its addition enables the precipitation of nucleic acids out of solution.
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3 M sodium acetate, pH 5.2 recipe
The recipe below can be used to prepare a 100 mL 3 M sodium acetate, pH 5.2 solution.
|Sodium acetate (anhydrous)||24.6 grams||3 M|
|MilliQ water||Up to 100 mL|
How to make 3 M sodium acetate, pH 5.2
- Weigh out 24.6 g of sodium acetate (anhydrous) and add to a 100 mL Duran bottle.
- Measure out 70 mL of MilliQ water and add to the Duran bottle.
- Dissolve the sodium acetate by adding a magnetic flea into the bottle and placing on a magnetic stirrer. It may take a few minutes to fully dissolve.
- Adjust the pH to 5.2 by adding glacial acetic acid.
- Top up the solution to 100 mL with MilliQ water.
- Optional: Filter the solution using a 0.2 uM filter membrane.
Storage of 3 M sodium acetate, pH 5.2 solution
Store 3 M sodium acetate, pH 5.2 solution at room temperature (+15oC – +25oC).
Sodium acetate is not classified as hazardous. However, always be sure to read the safety data sheet before use.