How To Make 5 M Sodium Chloride Solution

About sodium chloride solution

Sodium chloride (NaCl) solution, especially at a 5 M concentration, is a commonly used stock solution for a variety of solutions. One example of sodium chloride use is to precipitate nucleic acids during their extractions.

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5 M sodium chloride recipe

The recipe below can be used to prepare a 100 mL 5 M sodium chloride solution.

Reagent Weight/Volume Final concentration
Sodium chloride 29.2 grams 5 M
MilliQ water Up to 100 mL

How to make a 5 M sodium chloride solution

  1. Weigh out 29.2 g of sodium chloride and add to a 100 mL Duran bottle.
  2. Measure out 80 mL of MilliQ water and add to the Duran bottle.
  3. Dissolve the sodium chloride by adding a magnetic flea into the bottle and placing on a magnetic stirrer. It may take a few minutes to fully dissolve.
  4. Top up the solution to 100 mL with MilliQ water.
  5. To sterilise, autoclave the solution on a liquid cycle (20 min at 15 psi).
  6. Optional: Alternatively, filter the solution using a 0.2 uM filter membrane.

Storage of 5 M sodium chloride solution

Store 5 M sodium chloride solution at room temperature (+15oC – +25oC).

Safety

Sodium chloride is not classified as hazardous. However, always be sure to read the safety data sheet before use.

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