How To Make 5 M Sodium Chloride Solution

About sodium chloride solution

Sodium chloride (NaCl) solution, especially at a 5 M concentration, is a commonly used stock solution for a variety of solutions. One example of sodium chloride use is to precipitate nucleic acids during their extractions.

Download the recipe as a PDF

To download the 5 M sodium chloride recipe as a PDF then click here.

5 M sodium chloride recipe

The recipe below can be used to prepare a 100 mL 5 M sodium chloride solution.

Reagent Weight/Volume Final concentration
Sodium chloride 29.2 grams 5 M
MilliQ water Up to 100 mL

How to make a 5 M sodium chloride solution

  1. Weigh out 29.2 g of sodium chloride and add to a 100 mL Duran bottle.
  2. Measure out 80 mL of MilliQ water and add to the Duran bottle.
  3. Dissolve the sodium chloride by adding a magnetic flea into the bottle and placing on a magnetic stirrer. It may take a few minutes to fully dissolve.
  4. Top up the solution to 100 mL with MilliQ water.
  5. To sterilise, autoclave the solution on a liquid cycle (20 min at 15 psi).
  6. Optional: Alternatively, filter the solution using a 0.2 uM filter membrane.

Storage of 5 M sodium chloride solution

Store 5 M sodium chloride solution at room temperature (+15oC – +25oC).

Safety

Sodium chloride is not classified as hazardous. However, always be sure to read the safety data sheet before use.

Steven is the founder of Top Tip Bio. He is currently a Medical Writer and a former Postdoctoral Research Associate. Enjoyed the tutorial? Then let me know by leaving a comment below, or consider buying me a coffee.

LEAVE A REPLY

Please enter your comment!
Please enter your name here