About sodium chloride solution
Sodium chloride (NaCl) solution, especially at a 5 M concentration, is a commonly used stock solution for a variety of solutions. One example of sodium chloride use is to precipitate nucleic acids during their extractions.
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5 M sodium chloride recipe
The recipe below can be used to prepare a 100 mL 5 M sodium chloride solution.
| Reagent | Weight/Volume | Final concentration |
| Sodium chloride | 29.2 grams | 5 M |
| MilliQ water | Up to 100 mL |
How to make a 5 M sodium chloride solution
- Weigh out 29.2 g of sodium chloride and add to a 100 mL Duran bottle.
- Measure out 80 mL of MilliQ water and add to the Duran bottle.
- Dissolve the sodium chloride by adding a magnetic flea into the bottle and placing on a magnetic stirrer. It may take a few minutes to fully dissolve.
- Top up the solution to 100 mL with MilliQ water.
- To sterilise, autoclave the solution on a liquid cycle (20 min at 15 psi).
- Optional: Alternatively, filter the solution using a 0.2 uM filter membrane.
Storage of 5 M sodium chloride solution
Store 5 M sodium chloride solution at room temperature (+15oC – +25oC).
Safety
Sodium chloride is not classified as hazardous. However, always be sure to read the safety data sheet before use.
