About TE buffer
TE buffer (Tris-EDTA) is a commonly used buffer solution for resuspending and storing nucleic acids, especially DNA. The Tris solution keeps the DNA soluble in water while EDTA, a chelator of cations such as magnesium, protects nucleic acids against enzymatic degradation.
The recipe below is to create a 100 mL TE buffer solution.
|1M Tris-Cl (pH 8.0)||1 mL||10 mM|
|0.5M EDTA (pH 8.0)||0.2 mL||1 mM|
|Distilled H2O||98.8 mL|
How to make TE buffer
- Measure out 1 mL 1M Tris-Cl (pH 8.0) and add to a 100 mL Duran bottle.
- Measure out 0.2 mL 0.5M EDTA (pH 8.0) and add to the Duran bottle.
- Top up the solution to 100 mL by adding 98.8 mL of distilled water.
- Place the lid on the bottle and invert a few times to mix.
- To sterilise, autoclave the solution on a liquid cycle (20 min at 15 psi).
Storage of TE buffer
Store TE buffer at room temperature (+15oC – +25oC).
TE buffer is not classified as hazardous. However, always be sure to read the TE buffer safety data sheet before use.