PCR

Find guides, protocols and helpful tips when performing the polymerase chain reaction (PCR).

Bisulfite pyrosequencing pyrogram

How Does Bisulfite Pyrosequencing Work?

What is bisulfite pyrosequencing? Bisulfite, also referred to as bisulphite, pyrosequencing is a technique used to quantify DNA methylation levels on DNA at a single-base...

What Is The Polymerase Chain Reaction (PCR)?

What is the Polymerase Chain Reaction (PCR)? PCR is a molecular technique frequently applied to amplify regions of interest in DNA, resulting in millions or...
Pfaffl results graph

How To Perform The Pfaffl Method For qPCR

What is the Pfaffl method? The Pfaffl method, named after it's curator Michael Pfaffl, is used to calculate relative gene expression data while accounting for...

How To Make TE Buffer pH 8.0

About TE buffer TE buffer (Tris-EDTA) is a commonly used buffer solution for resuspending and storing nucleic acids, especially DNA. The Tris solution keeps the...

How To Make TBE Buffer (10x)

About TBE buffer TBE buffer, named so because of the of the three ingredients of Tris base, Boric acid and EDTA, is a solution commonly...

13 Free PCR Primer Design Programs

Gone are the days where you have to design primers by hand. Instead, we have a plethora of excellent resources available that utilises complex...

How To Calculate PCR Primer Efficiencies

Why bother with PCR primer efficiencies? Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain...
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How To Perform The Delta-Delta Ct Method

What is the delta-delta Ct method? The delta-delta Ct method, also known as the 2-∆∆Ct method, is a simple formula used in order to calculate...
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How To Create Real-Time PCR Primers Using Primer-BLAST

Primer-BLAST Primer-BLAST is NCBI’s free online primer design platform which designs PCR primers using the Primer3 system and simultaneously checks the likelihood that the primers...

How To Dilute New PCR Primers

So you have designed and ordered your primers and now they have finally arrived. But how do you prepare lyophilised (freeze-dried powder form) oligo...

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