Maths

Standard curve BCA assay

How To Create A Linear Standard Curve In Excel

In this guide I will explain how to create a linear standard curve using Microsoft Excel and how to use it to calculate unknown...

How To Use The Standard Error Formula

In this guide I will explain what the standard error formula is and how to use it to work out the standard error through...

How To Calculate The Coefficient Of Variation (CV)

What is the coefficient of variation? The coefficient of variation (CV) is a measure of precision from repeated measures. Within the lab, it is mainly...

How To Calculate PCR Primer Efficiencies

Why bother with PCR primer efficiencies? Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain...
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The C1V1 = C2V2 Equation Explained

The simple formula of C1V1 = C2V2 is a lifesaver for bioscience researchers in the lab who are wanting to do dilutions. Here I...
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How To Perform The Delta-Delta Ct Method

What is the delta-delta Ct method? The delta-delta Ct method, also known as the 2-∆∆Ct method, is a simple formula used in order to calculate...

How To Dilute New PCR Primers

In this article, I will explain how to easily prepare PCR stock primers and how to dilute them into a working primer solution. So you...