6 Tips For Using The Nanodrop To Measure DNA/RNA

Personally, I believe the Nanodrop is one of the most important, underrated pieces of equipment in the lab. By using just 1 μL of...

Guide To Sequencing On The Qiagen Q24 PyroMark

In this extensive guide, I will explain how to perform pyrosequencing on Qiagen's Q24 PyroMark Advanced system. To do this you need to have...

What Is The Polymerase Chain Reaction (PCR)?

What is the Polymerase Chain Reaction (PCR)? PCR is a molecular technique frequently applied to amplify regions of interest in DNA, resulting in millions or...

How To Make TE Buffer pH 8.0

About TE buffer TE buffer (Tris-EDTA) is a commonly used buffer solution for resuspending and storing nucleic acids, especially DNA. The Tris solution keeps the...

Guide To Agarose Gel Electrophoresis

If you are using DNA or doing PCR’s in your research, then you can guarantee you need to make up agarose gels at some...

How To Dilute New PCR Primers

So you have designed and ordered your primers and now they have finally arrived. But how do you prepare lyophilised (freeze-dried powder form) oligo...

How To Perform The Delta-Delta Ct Method

What is the delta-delta Ct method? The delta-delta Ct method, also known as the 2-∆∆Ct method, is a simple formula used in order to calculate...

How To Analyse ChIP qPCR Data

There are a few ways in which you can analyse chromatin immunoprecipitation (ChIP) data acquired from quantitative real-time polymerase chain reaction (qPCR). Two of...

How To Make TBE Buffer (10x)

About TBE buffer TBE buffer, named so because of the of the three ingredients of Tris base, Boric acid and EDTA, is a solution commonly...

5 Vital Tips For Perfect RNA Extraction Using TRIzol

RNA extraction using TRIzol is one of the most common molecular biology techniques used in the lab. Compared to spin column methods, it requires...

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