Guide To Sequencing On The Qiagen Q24 PyroMark

In this extensive guide, I will explain how to perform pyrosequencing on Qiagen's Q24 PyroMark Advanced system. To do this you need to have...

5 Vital Tips For Perfect RNA Extraction Using TRIzol

RNA extraction using TRIzol is one of the most common molecular biology techniques used in the lab. Compared to spin column methods, it requires...

How To Create A CpG Assay Using PyroMark Assay Design

To be able to design a CpG assay using the PyroMark Assay Design software, you first need your DNA sequence of interest that contains...

How To Make 0.5M EDTA pH 8.0

About EDTA solution Ethylenediaminetetraacetic acid (EDTA) solution, at 0.5 M pH 8.0, is a commonly used solution utilised as a ligand and chelating agent. EDTA...

6 Tips For Using The Nanodrop To Measure DNA/RNA

Personally, I believe the Nanodrop is one of the most important, underrated pieces of equipment in the lab. By using just 1 μL of...

Guide To Agarose Gel Electrophoresis

If you are using DNA or doing PCR’s in your research, then you can guarantee you need to make up agarose gels at some...

How To Analyse ChIP qPCR Data

There are a few ways in which you can analyse chromatin immunoprecipitation (ChIP) data acquired from quantitative real-time polymerase chain reaction (qPCR). Two of...
qPCR amplification plot annotated

What Is A Cycle Threshold (Ct) Value In qPCR?

In this article, I will explain what is meant by a Ct value in quantification (real-time) PCR (qPCR). I will also discuss the difference...

How To Calculate PCR Primer Efficiencies

Why bother with PCR primer efficiencies? Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain...

How To Make TE Buffer pH 8.0

About TE buffer TE buffer (Tris-EDTA) is a commonly used buffer solution for resuspending and storing nucleic acids, especially DNA. The Tris solution keeps the...

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