How To Analyse ChIP qPCR Data
There are a few ways in which you can analyse chromatin immunoprecipitation (ChIP) data acquired from quantitative real-time polymerase chain reaction (qPCR). Two of...
What Is ROX And Why Use It In qPCR?
To ROX or not to ROX? That is the question. I frequently see people confused about what ROX is and why it is used...
8 Tips For Using The Nanodrop To Measure DNA/RNA
Personally, I believe the Nanodrop is one of the most important, underrated pieces of equipment in the lab. By using just 1 μL of...
What Is A GC Clamp In PCR Primers?
In this article, I will explain what a GC clamp is in relation to PCR primer design and why they may be useful to...
How To Dilute New PCR Primers
In this article, I will explain how to easily prepare PCR stock primers and how to dilute them into a working primer solution.
Mastering qPCR
A...
How To Perform The Delta-Delta Ct Method
What is the delta-delta Ct method?
The delta-delta Ct method, also known as the 2-∆∆Ct method, is a simple formula used in order to calculate...
5 Vital Tips For Perfect RNA Extraction Using TRIzol
RNA extraction using TRIzol is one of the most common molecular biology techniques used in the lab. Compared to spin column methods, it requires...
Guide To Agarose Gel Electrophoresis
If you are using DNA or doing PCR’s in your research, then you can guarantee you need to make up agarose gels at some...
Load An Agarose Gel In Minutes With Parafilm
When you come to load an agarose gel with your DNA/PCR samples, you will first need to mix them with a loading buffer. The...
How To Calculate DNA Copy Number For qPCR
In this article, I will show you how to calculate the DNA copy number for qPCR, by using the known amount and length of...
