Load An Agarose Gel In Minutes With Parafilm
When you come to load an agarose gel with your DNA/PCR samples, you will first need to mix them with a loading buffer. The...
Guide To Sequencing On The Qiagen Q24 PyroMark
In this extensive guide, I will explain how to perform pyrosequencing on Qiagen's Q24 PyroMark Advanced system. To do this you need to have...
8 Tips For Using The Nanodrop To Measure DNA/RNA
Personally, I believe the Nanodrop is one of the most important, underrated pieces of equipment in the lab. By using just 1 μL of...
How To Perform The Pfaffl Method For qPCR
What is the Pfaffl method?
The Pfaffl method, named after it's curator Michael Pfaffl, is used to calculate relative gene expression data while accounting for...
How To Analyse ChIP qPCR Data
There are a few ways in which you can analyse chromatin immunoprecipitation (ChIP) data acquired from quantitative real-time polymerase chain reaction (qPCR). Two of...
How To Dilute New PCR Primers
In this article, I will explain how to easily prepare PCR stock primers and how to dilute them into a working primer solution.
Mastering qPCR
A...
The Hardy-Weinberg Equations And How To Use Them
What is the Hardy-Weinberg principle?
The Hardy-Weinberg principle, also referred to as the Hardy-Weinberg equilibrium, is a set of 5 assumptions which when satisfied can...
How To Calculate PCR Primer Efficiencies
Why bother with PCR primer efficiencies?
Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain...
Qubit vs Nanodrop: Which Is Better?
When it comes to quantifying nucleic acids, there are two preferred main platforms: the Qubit and the Nanodrop. Both work slightly differently and have...
How To Make TBE Buffer
About TBE buffer
TBE buffer, named so because of the three ingredients of Tris base, Boric acid and EDTA, is a solution commonly used as...
