8 Tips For Using The Nanodrop To Measure DNA/RNA
Personally, I believe the Nanodrop is one of the most important, underrated pieces of equipment in the lab. By using just 1 μL of...
Guide To Agarose Gel Electrophoresis
If you are using DNA or doing PCR’s in your research, then you can guarantee you need to make up agarose gels at some...
The Hardy-Weinberg Equations And How To Use Them
What is the Hardy-Weinberg principle?
The Hardy-Weinberg principle, also referred to as the Hardy-Weinberg equilibrium, is a set of 5 assumptions which when satisfied can...
How To Calculate PCR Primer Efficiencies
Why bother with PCR primer efficiencies?
Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain...
What Is The Polymerase Chain Reaction (PCR)?
What is the Polymerase Chain Reaction (PCR)?
PCR is a molecular technique frequently applied to amplify regions of interest in DNA, resulting in millions or...
Load An Agarose Gel In Minutes With Parafilm
When you come to load an agarose gel with your DNA/PCR samples, you will first need to mix them with a loading buffer. The...
How Does Bisulfite Pyrosequencing Work?
What is bisulfite pyrosequencing?
Bisulfite, also referred to as bisulphite, pyrosequencing is a technique used to quantify DNA methylation levels on DNA at a single-base...
The Features Of A Good QPCR Primer Pair
In this guide, I will give you some of my thoughts on features of a good qPCR primer pair. I will explain what to...
Random Primers Or Oligo(dT) Primers For cDNA Synthesis?
Having extracted and assessed the total RNA from your samples, you now have the task of creating the first strand complementary DNA (cDNA). Sounds...
How To Perform The Pfaffl Method For qPCR
What is the Pfaffl method?
The Pfaffl method, named after it's curator Michael Pfaffl, is used to calculate relative gene expression data while accounting for...
