Techniques

Bisulfite pyrosequencing pyrogram

How Does Bisulfite Pyrosequencing Work?

What is bisulfite pyrosequencing? Bisulfite, also referred to as bisulphite, pyrosequencing is a technique used to quantify DNA methylation levels on DNA at a single-base...
Random primers, oligo(dT) and gene-specific primers for cDNA synthesis

Random Primers Or Oligo(dT) Primers For cDNA Synthesis?

Having extracted and assessed the total RNA from your samples, you now have the task of creating the first strand complementary DNA (cDNA). Sounds...

How To Analyse ChIP qPCR Data

There are a few ways in which you can analyse chromatin immunoprecipitation (ChIP) data acquired from quantitative real-time polymerase chain reaction (qPCR). Two of...
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How To Perform The Delta-Delta Ct Method

What is the delta-delta Ct method? The delta-delta Ct method, also known as the 2-∆∆Ct method, is a simple formula used in order to calculate...

Load An Agarose Gel In Minutes With Parafilm

When you come to load an agarose gel with your DNA/PCR samples, you will first need to mix them with a loading buffer. The...

Guide To Sequencing On The Qiagen Q24 PyroMark

In this extensive guide, I will explain how to perform pyrosequencing on Qiagen's Q24 PyroMark Advanced system. To do this you need to have...

qPCR Primer Efficiency Calculator

Below is a PCR primer efficiency calculator. All that is needed for the calculator is the slope of the standard curve during the serial...
Qubit vs Nanodrop featured image

Qubit vs Nanodrop: Which Is Better?

When it comes to quantifying nucleic acids, there are two preferred main platforms: the Qubit and the Nanodrop. Both work slightly differently and have...

How To Dilute New PCR Primers

In this article, I will explain how to easily prepare PCR stock primers and how to dilute them into a working primer solution. So you...
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How To Create Real-Time PCR Primers Using Primer-BLAST

Primer-BLAST Primer-BLAST is NCBI’s free online primer design platform which designs PCR primers using the Primer3 system and simultaneously checks the likelihood that the primers...

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