Techniques

Random primers, oligo(dT) and gene-specific primers for cDNA synthesis

Random Primers Or Oligo(dT) Primers For cDNA Synthesis?

Having extracted and assessed the total RNA from your samples, you now have the task of creating the first strand complementary DNA (cDNA). Sounds...

Load An Agarose Gel In Minutes With Parafilm

When you come to load an agarose gel with your DNA/PCR samples, you will first need to mix them with a loading buffer. The...

How To Make TBE Buffer

About TBE buffer TBE buffer, named so because of the three ingredients of Tris base, Boric acid and EDTA, is a solution commonly used as...

How To Analyse qPCR Results With Multiple Reference Genes

A question that I often come across for those who are calculating relative gene expression values in qPCR is, how to go about using...

How To Create A CpG Assay Using PyroMark Assay Design

To be able to design a CpG assay using the PyroMark Assay Design software, you first need your DNA sequence of interest that contains...

qPCR Primer Efficiency Calculator

Below is a PCR primer efficiency calculator. All that is needed for the calculator is the slope of the standard curve during the serial...

What Is The Polymerase Chain Reaction (PCR)?

What is the Polymerase Chain Reaction (PCR)? PCR is a molecular technique frequently applied to amplify regions of interest in DNA, resulting in millions or...
Nanodrop curve overview

The Nanodrop Results Explained

The Nanodrop is one of my favourite pieces of equipment in the lab. In exchange for just 1 μL of sample, not only do...

DNA Copy Number Online Calculator

Below is a calculator that can be used to estimate the number of copies of DNA, by simply entering the amount used and the...
GC clamp example

What Is A GC Clamp In PCR Primers?

In this article, I will explain what a GC clamp is in relation to PCR primer design and why they may be useful to...

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