Techniques

Nanodrop curve overview

The Nanodrop Results Explained

The Nanodrop is one of my favourite pieces of equipment in the lab. In exchange for just 1 μL of sample, not only do...

Load An Agarose Gel In Minutes With Parafilm

When you come to load an agarose gel with your DNA/PCR samples, you will first need to mix them with a loading buffer. The...

8 Tips For Using The Nanodrop To Measure DNA/RNA

Personally, I believe the Nanodrop is one of the most important, underrated pieces of equipment in the lab. By using just 1 μL of...

5 Vital Tips For Perfect RNA Extraction Using TRIzol

RNA extraction using TRIzol is one of the most common molecular biology techniques used in the lab. Compared to spin column methods, it requires...

How To Make 0.5M EDTA pH 8.0

About EDTA solution Ethylenediaminetetraacetic acid (EDTA) solution, at 0.5 M pH 8.0, is a commonly used solution utilised as a ligand and chelating agent. EDTA...

Guide To Agarose Gel Electrophoresis

If you are using DNA or doing PCR’s in your research, then you can guarantee you need to make up agarose gels at some...
qPCR amplification plot annotated

What Is A Cycle Threshold (Ct) Value In qPCR?

In this article, I will explain what is meant by a Ct value in quantification (real-time) PCR (qPCR). I will also discuss the difference...

How To Analyse qPCR Results With Multiple Reference Genes

A question that I often come across for those who are calculating relative gene expression values in qPCR is, how to go about using...
Hardy-Weinberg equation featured image

The Hardy-Weinberg Equations And How To Use Them

What is the Hardy-Weinberg principle? The Hardy-Weinberg principle, also referred to as the Hardy-Weinberg equilibrium, is a set of 5 assumptions which when satisfied can...

How To Calculate PCR Primer Efficiencies

Why bother with PCR primer efficiencies? Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain...

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