Techniques

5 Vital Tips For Perfect RNA Extraction Using TRIzol

RNA extraction using TRIzol is one of the most common molecular biology techniques used in the lab. Compared to spin column methods, it requires...
Pfaffl results graph

How To Perform The Pfaffl Method For qPCR

What is the Pfaffl method? The Pfaffl method, named after it's curator Michael Pfaffl, is used to calculate relative gene expression data while accounting for...
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How To Perform The Delta-Delta Ct Method

What is the delta-delta Ct method? The delta-delta Ct method, also known as the 2-∆∆Ct method, is a simple formula used in order to calculate...
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How To Create Real-Time PCR Primers Using Primer-BLAST

Primer-BLAST Primer-BLAST is NCBI’s free online primer design platform which designs PCR primers using the Primer3 system and simultaneously checks the likelihood that the primers...

Guide To Agarose Gel Electrophoresis

If you are using DNA or doing PCR’s in your research, then you can guarantee you need to make up agarose gels at some...

8 Tips For Using The Nanodrop To Measure DNA/RNA

Personally, I believe the Nanodrop is one of the most important, underrated pieces of equipment in the lab. By using just 1 μL of...

Guide To Sequencing On The Qiagen Q24 PyroMark

In this extensive guide, I will explain how to perform pyrosequencing on Qiagen's Q24 PyroMark Advanced system. To do this you need to have...
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The Hardy-Weinberg Equations And How To Use Them

What is the Hardy-Weinberg principle? The Hardy-Weinberg principle, also referred to as the Hardy-Weinberg equilibrium, is a set of 5 assumptions which when satisfied can...

What Is The Polymerase Chain Reaction (PCR)?

What is the Polymerase Chain Reaction (PCR)? PCR is a molecular technique frequently applied to amplify regions of interest in DNA, resulting in millions or...
Agarose gel

Reasons Why Your PCR Reaction Does Not Work

We have all been there. You have been performing PCRs perfectly for weeks/months on end but now, for some bizarre reason, you see …...