Techniques

How To Create A CpG Assay Using PyroMark Assay Design

To be able to design a CpG assay using the PyroMark Assay Design software, you first need your DNA sequence of interest that contains...

5 Vital Tips For Perfect RNA Extraction Using TRIzol

RNA extraction using TRIzol is one of the most common molecular biology techniques used in the lab. Compared to spin column methods, it requires...

Guide To Agarose Gel Electrophoresis

If you are using DNA or doing PCR’s in your research, then you can guarantee you need to make up agarose gels at some...
Pfaffl results graph

How To Perform The Pfaffl Method For qPCR

What is the Pfaffl method? The Pfaffl method, named after it's curator Michael Pfaffl, is used to calculate relative gene expression data while accounting for...

What Is The Polymerase Chain Reaction (PCR)?

What is the Polymerase Chain Reaction (PCR)? PCR is a molecular technique frequently applied to amplify regions of interest in DNA, resulting in millions or...

How To Make 0.5M EDTA pH 8.0

About EDTA solution Ethylenediaminetetraacetic acid (EDTA) solution, at 0.5 M pH 8.0, is a commonly used solution utilised as a ligand and chelating agent. EDTA...
qPCR amplification plot annotated

What Is A Cycle Threshold (Ct) Value In qPCR?

In this article, I will explain what is meant by a Ct value in quantification (real-time) PCR (qPCR). I will also discuss the difference...
Random primers, oligo(dT) and gene-specific primers for cDNA synthesis

Random Primers Or Oligo(dT) Primers For cDNA Synthesis?

Having extracted and assessed the total RNA from your samples, you now have the task of creating the first strand complementary DNA (cDNA). Sounds...

How To Analyse ChIP qPCR Data

There are a few ways in which you can analyse chromatin immunoprecipitation (ChIP) data acquired from quantitative real-time polymerase chain reaction (qPCR). Two of...

How To Make TBE Buffer

About TBE buffer TBE buffer, named so because of the three ingredients of Tris base, Boric acid and EDTA, is a solution commonly used as...