How To Make TBE Buffer
About TBE buffer
TBE buffer, named so because of the three ingredients of Tris base, Boric acid and EDTA, is a solution commonly used as...
Guide To Sequencing On The Qiagen Q24 PyroMark
In this extensive guide, I will explain how to perform pyrosequencing on Qiagen's Q24 PyroMark Advanced system. To do this you need to have...
5 Vital Tips For Perfect RNA Extraction Using TRIzol
RNA extraction using TRIzol is one of the most common molecular biology techniques used in the lab. Compared to spin column methods, it requires...
13 Free PCR Primer Design Programs
Gone are the days where you have to design primers by hand. Instead, we have a plethora of excellent resources available that utilises complex...
How To Create A CpG Assay Using PyroMark Assay Design
To be able to design a CpG assay using the PyroMark Assay Design software, you first need your DNA sequence of interest that contains...
Load An Agarose Gel In Minutes With Parafilm
When you come to load an agarose gel with your DNA/PCR samples, you will first need to mix them with a loading buffer. The...
How To Calculate PCR Primer Efficiencies
Why bother with PCR primer efficiencies?
Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain...
Guide To Agarose Gel Electrophoresis
If you are using DNA or doing PCR’s in your research, then you can guarantee you need to make up agarose gels at some...
How To Create Real-Time PCR Primers Using Primer-BLAST
Primer-BLAST
Primer-BLAST is NCBI’s free online primer design platform which designs PCR primers using the Primer3 system and simultaneously checks the likelihood that the primers...
8 Tips For Using The Nanodrop To Measure DNA/RNA
Personally, I believe the Nanodrop is one of the most important, underrated pieces of equipment in the lab. By using just 1 μL of...